Figure 3
From: A requirement for hedgehog signaling in thyroid hormone-induced postembryonic intestinal remodeling
Cyclopamine inhibits Shh target gene expression. Tadpoles at stage 58 were treated as in Figure 2 until they reached stage 62. Total intestinal RNA was isolated for qRT-PCR analysis of the expression of Shh target genes BMP4 [51] (A), Snai2 [52] (B) and Twist1 [52] (C). ** indicate significant difference between the cyclopamine group and the other groups (p < 0.001).