The changes of cardiomyocytes apoptosis and related factors with apoptosis. (A). TUNEL staining detected cardiomyocytes apoptosis. Nuclei with brown staining indicate TUNEL positive cells (400×). The cells of arrow are apoptotic cells. The level of Bcl-2 (B), caspase-3 (C) and caspase-9 mRNA (D). The level of Bcl-2, caspase-3 and caspase-9 mRNA was tested using Real-Time PCR. The data were normalized to the GAPDH. (E). Release of Cyt c. The intensity of each band was quantified by densitometry, and data were normalized to the GAPDH signal. All data were from four independent experiments. * p < 0.05 vs. control group; # p < 0.05 vs. I/R group; & p < 0.05 vs. PC group; $ p < 0.05 vs. I/R + NaHS group; § p < 0.05 vs. PC + NaHS group.