Overexpression of Nanog promotes β-catenin phosphorylation. The protein of total and phosphorylated GSK-3β,total and phosphorylated β-catenin were detected (A). The ratio of phosphorylated GSK-3β to total GSK-3β was an indicator to inactivation of β-catenin (B). The bands of β-catenin protein in each group were analyzed quantitatively (C and D). The ratio of phosphorylated β-catenin to total β-catenin was regarded as the inactive β-catenin (E). (*P < 0.05) (Con: control; SP: substance P treated group; LV-Nanog: transduced group).