Expression of β-catenin and Nanog in EpSCs treated with Wnt antagonist, Dkk-1. Real-time PCR analysis of β-catenin mRNA (A) and Nanog mRNA (B) were obtained at different time points (day 1- day 12) in the control, SP treated group and SP with Dkk-1 treated group. The result showed that there were significant differences between the SP group and SP with Dkk-1 group on or after day 3 (*P < 0.05). At day 12, protein of Nanog and β-catenin were also detected (C). The bands of both proteins in each group were analyzed quantitatively (D and E). Werstern blot results were similar to q-PCR results (Con: control; SP: SP treated group; SP + Dkk-1: SP with Dkk-1 treated group).