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Table 2 Sequences of primers and PCR conditions

From: Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro

  Primer sequence Product size (bp) Ta (°C)/cycles
RAGE S: 5′-CTGGTGTTCCCAATAAGG-3′ 372 58.5/38
  AS: 5′-AGGTCAGGGTTACGGTT-3′   
GAPDH S: 5′-ACCACAGTCCATGCCATCAC-3′ 452 56.0/26
  AS: 5′-TCCACCACCCTGTTGCTGTA-3′   
  1. S, sense; AS, antisense; Ta, annealing temperature.