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Table 2 Sequences of primers and PCR conditions

From: Effect of advanced glycosylation end products on apoptosis in human adipose tissue-derived stem cells in vitro

 

Primer sequence

Product size (bp)

Ta (°C)/cycles

RAGE

S: 5′-CTGGTGTTCCCAATAAGG-3′

372

58.5/38

 

AS: 5′-AGGTCAGGGTTACGGTT-3′

  

GAPDH

S: 5′-ACCACAGTCCATGCCATCAC-3′

452

56.0/26

 

AS: 5′-TCCACCACCCTGTTGCTGTA-3′

  
  1. S, sense; AS, antisense; Ta, annealing temperature.