Skip to main content

Advertisement

Table 1 Up-regulated proteins were identified by a SILAC-based proteomic approach

From: Quantitative proteomic analysis of single or fractionated radiation-induced proteins in human breast cancer MDA-MB-231 cells

no Accession No Gene symbol Identified proteins Fold P value * S 1 S 2 F 1 F 2
Commonly up-regulated proteins
1 IPI00022418 FN1 Isoform 1 of Fibronectin 6.525 0.0000 1.7 1.9 17.7 4.8
2 IPI00011229 CTSD Cathepsin D 3.200 0.0000 2.9 3.2 3.4 3.3
3 IPI00026314 GSN Isoform 1 of Gelsolin 2.375 0.0000 3 2.6 2 1.9
4 IPI00020632 ASS1 Argininosuccinate synthase 2.175 0.0007 2.3 2.1 2.4 1.9
5 IPI00024915 PRDX5 Isoform Mitochondrial of Peroxiredoxin-5, mitochondrial 2.025 0.0606 2.9 1.8 1.5 1.9
6 IPI00019157 CSPG4 Chondroitin sulfate proteoglycan 4 1.900 0.3173 1.4 1 2 3.2
7 IPI00218474 ENO3 Isoform 1 of Beta-enolase 1.900 0.0145 1 1 4.6 1
8 IPI00005707 MRC2 C-type mannose receptor 2 1.850 0.0074 1.3 1.2 2.5 2.4
9 IPI00940829 NEDD4 Isoform 4 of E3 ubiquitin-protein ligase NEDD4 1.850 0.0004 1.3 1.3 2.4 2.4
10 IPI00021048 MYOF Isoform 1 of Myoferlin 1.800 0.0000 1.8 1.7 2.4 1.3
11 IPI00021812 AHNAK Neuroblast differentiation-associated protein AHNAK 1.800 0.0047 1.8 2.2 1.6 1.6
12 IPI00020557 LRP1 Prolow-density lipoprotein receptor-related protein 1 1.800 0.0000 1.4 1.4 1.8 2.6
13 IPI00021766 RTN4 Isoform 1 of Reticulon-4 1.725 0.0019 1.6 1.5 1.7 2.1
14 IPI00006663 ALDH2 Aldehyde dehydrogenase, mitochondrial 1.625 0.0262 2.1 1.8 1.4 1.2
Single (10 Gy)
1 IPI00011229 CTSD Cathepsin D 3.05   2.9 3.2   
2 IPI00026314 GSN Isoform 1 of Gelsolin 2.8   3 2.6   
3 IPI00024915 PRDX5 Isoform Mitochondrial of Peroxiredoxin-5, mitochondrial 2.35   2.9 1.8   
4 IPI00020632 ASS1 Argininosuccinate synthase 2.2   2.3 2.1   
5 IPI00021812 AHNAK Neuroblast differentiation-associated protein AHNAK 2   1.8 2.2   
6 IPI00006663 ALDH2 Aldehyde dehydrogenase, mitochondrial 1.95   2.1 1.8   
7 IPI00022418 FN1 Isoform 1 of Fibronectin 1.8   1.7 1.9   
8 IPI00021048 MYOF Isoform 1 of Myoferlin 1.75   1.8 1.7   
9 IPI00021766 RTN4 Isoform 1 of Reticulon-4 1.55   1.6 1.5   
10 IPI00216694 PLS3 Plastin-3 1.55   1.6 1.5   
11 IPI00382844 ACO2 Aconitase (Fragment) 1.55   1.5 1.6   
12 IPI00013860 HIBADH 3-hydroxyisobutyrate dehydrogenase, mitochondrial 1.55   1.5 1.6   
13 IPI00018350 MCM5 DNA replication licensing factor MCM5 1.55   1.6 1.5   
14 IPI00098902 OGDH 2-oxoglutarate dehydrogenase, mitochondrial 1.5   1.5 1.5   
15 IPI00784156 AP2B1 Isoform 1 of AP-2 complex subunit beta 1.5   1.5 1.5   
16 IPI00456969 DYNC1H1 Cytoplasmic dynein 1 heavy chain 1 1.5   1.5 1.5   
Fraction (2 Gy × 5)
1 IPI00022418 FN1 Isoform 1 of Fibronectin 11.25     17.7 4.8
2 IPI00011229 CTSD Cathepsin D 3.35     3.4 3.3
3 IPI00218474 ENO3 Isoform 1 of Beta-enolase 2.8     4.6 1
4 IPI00019157 CSPG4 Chondroitin sulfate proteoglycan 4 2.6     2 3.2
5 IPI00005707 MRC2 C-type mannose receptor 2 2.45     2.5 2.4
6 IPI00940829 NEDD4 Isoform 4 of E3 ubiquitin-protein ligase NEDD4 2.4     2.4 2.4
7 IPI00020557 LRP1 Prolow-density lipoprotein receptor-related protein 1 2.2     1.8 2.6
8 IPI00216457 HIST2H2AA3 Histone H2A type 2-A 2.2     2.3 2.1
9 IPI00020632 ASS1 Argininosuccinate synthase 2.15     2.4 1.9
10 IPI00026314 GSN Isoform 1 of Gelsolin 1.95     2 1.9
11 IPI00018398 PSMC3 26S protease regulatory subunit 6A 1.95     2.8 1.1
12 IPI00021766 RTN4 Isoform 1 of Reticulon-4 1.9     1.7 2.1
13 IPI00021048 MYOF Isoform 1 of Myoferlin 1.85     2.4 1.3
14 IPI00293464 DDB1 DNA damage-binding protein 1 1.75     1.4 2.1
15 IPI00815770 SNX3 Isoform 1 of Sorting nexin-3 1.75     2.3 1.2
16 IPI00024915 PRDX5 Isoform Mitochondrial of Peroxiredoxin-5, mitochondrial 1.7     1.5 1.9
17 IPI00007682 ATP6V1A V-type proton ATPase catalytic subunit A 1.7     1.7 1.7
18 IPI00021812 AHNAK Neuroblast differentiation-associated protein AHNAK 1.6     1.6 1.6
19 IPI00010418 MYO1C Isoform 2 of Myosin-Ic 1.6     1.8 1.4
20 IPI00000005 NRAS GTPase NRas 1.6     1.6 1.6
21 IPI00006482 ATP1A1 Isoform Long of Sodium/potassium-transporting ATPase subunit alpha-1 1.6     1.4 1.8
22 IPI00023006 ACTC1 Actin, alpha cardiac muscle 1 1.6     2.1 1.1
23 IPI00221035 BTF3 Isoform 1 of Transcription factor BTF3 1.6     1.6 1.6
24 IPI00007402 IPO7 Importin-7 1.55     2.2 0.9
25 IPI00246058 PDCD6IP Programmed cell death 6-interacting protein 1.55     1.7 1.4
26 IPI00026689 CDK1 Putative uncharacterized protein DKFZp686L20222 1.5     1.5 1.5
27 IPI00001960 CLIC4 Chloride intracellular channel protein 4 1.5     1.4 1.6
  1. Proteins increased by an average of 1.5-fold or more are listed.
  2. *Statistical comparisons were performed using the paired z-test, and experimentally verified proteins are represented in bold. S: single-dose irradiation, F; fractionated-dose irradiation.