Figure 5

Increased nuclear translocation of Nrf2 by SWT or z-LIG. Immunocytochemistry of MCF-10A cells showed dose-dependent nuclear translocation of Nrf2 after treating with SWT or z-LIG for 24 hours. H₂O₂ (70 uM) and Resveratrol (10 uM) were used as positive controls. Cells were incubated with Nrf2 antibody (Alexa Fluor 594) after treatment and counterstained with Hoechst (5 uM) for nuclear staining, then visualized under a fluorescent microscope. All images were taken under 20X magnification. Images were merged using ImageJ software (National Institutes of Health). The experiment was repeated twice and obtained consistent results.