Figure 2

Effects of SWT on H ₂ O ₂ -induced apoptosis in MCF-10A cells. MCF-10A cells (3 × 10⁵ cells/well in a 6-well tissue culture plate) were pre-treated with SWT 2.56 or 4.0 mg/mL for two hour then exposed to 0.125, 0.25 or 0.5 mM of H₂O₂ for 24 hours. Cells were collected, washed with PBS and resuspended in 1X Annexin V Binding Buffer. Annexin V and/or Propidium iodide (PI) were added to the samples and analyzed by flow cytometry. Flow cytometry profile represents Annexin-V-FITC staining in x axis and PI in y axis. The numbers in each of the quadrant represent the percentage of viable and healthy (lower left), early apoptosis (lower right), necrosis (upper left) and late apoptosis (upper right). The experiment was repeated twice and obtained consistent results.