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Figure 2 | Cell & Bioscience

Figure 2

From: Development of a mouse monoclonal antibody for the detection of asymmetric dimethylarginine of Translocated in LipoSarcoma/FUsed in Sarcoma and its application in analyzing methylated TLS

Figure 2

In vitro methylation of the recombinant GST-TLS. GST-TLS was in vitro methylated using PRMT1 in the presence or absence of SAM (20 μM). Reaction products were analyzed by SDS-PAGE followed by western blotting with the indicated antibodies: anti-GST (top), 2B12 (middle), and anti-PRMT1 (bottom). Note that 2B12 specifically reacts with TLS methylated by PRMT1 only in the presence of SAM, and methylated TLS strongly associates with PRMT1.

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