ESE-1 and GP73 were elevated in the liver tissues of patients with HCC. Liver tissues of HCC patients (n = 5) were collected and fixed by formaldehyde. Hematoxylin–eosin (HE) staining was used to present the pathological changes in liver tissues. ESE-1 (red fluorescence) and GP73 (green fluorescence) expression levels were analyzed by conducting immunofluorescence. Nuclei (blue fluorescence) were counterstained with DAPI. The isotype-matched IgG was used as a negative control.