Effect of rhodopsin T17M on ubiquitin-proteasome system activity. (a) ARPE-19 cells stably expressing GFPU were incubated with or without MG132 for 2 h to block the proteasome. (b) ARPE-19 cells stably expressing GFPU were transiently transfected with rhodopsin T17M or WT. Lysates were immunoblotted with the antibody to GFP or myc. (c) Quantification of relative GFP level shown in (b). Data were presented as mean ± S.D.