Figure 2
From: Chemical chaperone 4-phenylbutyrate prevents endoplasmic reticulum stress induced by T17M rhodopsin
Knockdown of erasin and overexpression of p97/VCP-QQ inhibit rhodopsin turnover. ARPE-19 cells were transfected with myc-tagged rhodopsin T17M (a) or WT (b) expression constructs 48 h after transfection with erasin siRNAs. At 72 h post-knockdown, cells were treated with cycloheximide for the indicated time points and the proteins were detected by immunoblotting. ARPE-19 cells were cotransfected with HA-tagged p97/VCP-QQ and rhodopsin T17M (c). 36 h after co-transfection, cells were treated with cycloheximide for the indicated time points and the proteins were detected by immunoblotting. Quantification of relative level of remained WT or T17M rhodopsin was shown in right panels. Data were presented as mean ± S.D. **p < 0.01 compared to Control.