IL-25 induces up-regulation of cytokines in T84 colonic epithelial cells. T84 cells were cultured in a mixture of Ham’s F12 and Dulbecco’s modified Eagle’s medium containing 5% FBS. One million cells per well were seeded on a 6-well plate and incubated overnight, and then treated with IL-25 (50 ng/ml) for 24 hours in triplicates. Cells were collected for mRNA expression of cytokines by qPCR. Data are mean ± SEM and are representatives of two independent experiments. *P < 0.05 versus the respective vehicle.