Figure 3

Colonic expression of cytokines, mediators, and inflammatory cell markers. Mice genetically deficient in IL-25 and WT mice were given 3.2% DSS in drinking water for 7 days and euthanized at day 8. qPCR was performed to examine mRNA expression of IL-33 (A), pro-inflammatory cytokines/mediators (B), and inflammatory cell markers (C) in the colon. The fold changes are relative to the individual WT-VEH groups after normalization to 18 s rRNA. Data are mean ± SEM and are representatives of two independent experiments with five mice per group. *P < 0.05 versus the respective VEH; ϕ P < 0.05 versus respective WT-DSS.