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Figure 3 | Cell & Bioscience

Figure 3

From: Induction of ER stress-mediated apoptosis by ceramide via disruption of ER Ca2+ homeostasis in human adenoid cystic carcinoma cells

Figure 3

Fumonisin B1 inhibits ceramide-induced ER stress. (A) ACC-M and ACC-2 cells were seeded into 60 mm culture dishes and the next day cells were pretreated with 20 μM FB1. After 3 h incubation, 100 μM ceramide was added for further 12 h incubation in full medium. Total RNA was isolated for detection of XBP1 mRNA splicing using RT-PCR. The experiment was repeated three times and the representative result is shown. (B) Cells were treated as in A. After 20 μM FB1 treatment for 3 h, 100 μM ceramide was added for further 3 h incubation. Whole cell lysates were collected to analyze phosphorylated eIF2α expression. The experiment was repeated several times and the representative result is shown.

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