Figure 2

Haematoxylin and eosin staining images revealing morphological changes in the nuclear and cytoplasmic components in SNO cells. (A) Cells propogated in medium only, (B) cells exposed to dimethyl sulphoxide (DMSO) – vehicle control, (C) cells exposed to actinomycin D – positive control and (D) cells exposed to ESE-16 at a concentration of 0.2 μM. Cells propogated in medium and the vehicle-treated cells showed normal cell morphology, with the majority of the cells being in interphase. The positive control for apoptosis and the ESE-16 treated cells showed a decrease in cell density and morphological characteristics of apoptosis such as membrane blebbing and apoptotic bodies. The ESE-16 treated cells also showed an increase in the number of round cells with hypercondensed chromatin indicative of a metaphase block (Magnification: ×20).