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Figure 3 | Cell & Bioscience

Figure 3

From: When is a vesicle not just a vesicle: mitochondrial spheroids and mitochondrial autophagosomes

Figure 3

Effect of mitofusin-1 on mitochondrial vesicle formation. A. Mitofusin-1 and mitofusin-2 protein levels in LCC9 cells treated with 100 nM ICI or 10 µM Imatinib for 72 hours. B. Knockdown of mitofusin-1 in LCC9 cells was confirmed by Western blot hybridization and mitofusin-1 knockdown had no effect on either parkin expression or autophagosome formation. C. EM micrographs of LCC9 cells treated with control or mitofusin-1 siRNA. Arrows denotes mitochondrial forming vesicles. D. Autophagosomes were counted from EM images of LCC9 cells treated with control or mitofusin-1 siRNA. n = 10; *p > 0.05. E. Mitochondria forming vesicles were counted from EM images of LCC9 cells treated with control or mitofusin-1 siRNA. Data was graphed as % mitochondrial forming vesicles per image. n = 10; *p > 0.05.

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