From: Recent advances in hydrogen peroxide imaging for biological applications
Fluorescent probes | Excitation/Emission (nm) | Additional features | References | |||
---|---|---|---|---|---|---|
Abbreviation | Full name | Â | Detection mechanism | Intra-cellular | In vivo | Â |
PX-1 | Peroxyxanthone-1 | 350/440 | Boronate-deprotection mechanism | Yes | N/A | [31] |
FP-H2O2-NO | N/A | 400/460 | Response to H2O2, NO, or both | Yes | N/A | [32] |
DMACA | N/A | 400/484, 566 | Ratiometric imaging | Yes | N/A | [33] |
DPPEA-HC | 7-hydroxy-2-oxo-N-(2-(diphenylphosphino)ethyl)-2H-chromene-3-carboxamide | 403/449 | PET control | N/A | N/A | [34] |
PL-1 | Peroxy Lucifer 1 | 410/475, 540 | Boronate-deprotection mechanism; Ratiometric imaging | Yes | N/A | [35] |
RPF-1 | Ratio Peroxyfluor 1 | 420/464, 517 | Boronate-deprotection mechanism; Ratiometric imaging | N/A | N/A | [36] |
PF-1 | Peroxyfluor-1 | 450/>460 | Boronate-deprotection mechanism | Yes | N/A | |
PF-2 | Peroxyfluor-2 | 475/511 | Boronate-deprotection mechanism | Yes | Yes | |
PF6-AM | Peroxyfluor-6 acetoxymethyl ester | 482/517 | Boronate-deprotection mechanism; High cellular permeability | Yes | N/A | |
NBzF | N/A | 490/525 | PET control | Yes | N/A | [40] |
PF-3 | Peroxyfluor-3 | 492/515 | Boronate-deprotection mechanism | Yes | N/A | [38] |
RF-1 | Redoxfluor 1 | 495/503 | Boronate-deprotection mechanism | Yes | N/A | [41] |
MitoPY1 | Mitochondrial Peroxy Yellow 1 | 510/528 | Boronate-deprotection mechanism; Mitochondria-targeted | Yes | N/A | |
PY1-ME | Peroxy Yellow 1 Methyl-Ester | 515/540 | Boronate-deprotection mechanism | Yes | N/A | [44] |
(NucPE) 1 | Nuclear Peroxy Emerald 1 | 514/540 | Boronate-deprotection mechanism; Nuclear localized | Yes | Yes | [45] |
PY1 | Peroxy Yellow 1 | 519/548 | Boronate-deprotection mechanism | Yes | N/A | |
PO1 | Peroxy Orange 1 | 540/565 | Boronate-deprotection mechanism | Yes | N/A | |
Amplex Red | Amplex Red | 571/581 | Conjunction with horseradish peroxidase | N/A | Yes | |
PR-1 | Peroxy Red 1 | ~575/585 | Boronate-deprotection mechanism | Yes | N/A | [31] |
PCL-1 | Peroxy Caged Luciferin 1 | NA/612 | Boronate-deprotection mechanism; Bioluminescence imaging | N/A | Yes | |
PCL-2 | Peroxy Caged Luciferin 2 | Â | Boronate-deprotection mechanism; Bioluminescence imaging | N/A | Yes | [48] |
QCy7 | Quinone Cy-7 | 595/635 | Cyanine-based probe | N/A | Yes | [49] |
PN1 | Peroxy Naphthalene 1 | TPM750nm | Boronate-deprotection mechanism | N/A | Yes | [50] |
SHP-Mito | N/A | TPM750nm | Boronate-deprotection mechanism Mitochondria-targeted | Yes | Yes | [51] |