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Table 1 Small molecule probes for H 2 O 2 imaging

From: Recent advances in hydrogen peroxide imaging for biological applications

Fluorescent probes Excitation/Emission (nm) Additional features References
Abbreviation Full name   Detection mechanism Intra-cellular In vivo  
PX-1 Peroxyxanthone-1 350/440 Boronate-deprotection mechanism Yes N/A [31]
FP-H2O2-NO N/A 400/460 Response to H2O2, NO, or both Yes N/A [32]
DMACA N/A 400/484, 566 Ratiometric imaging Yes N/A [33]
DPPEA-HC 7-hydroxy-2-oxo-N-(2-(diphenylphosphino)ethyl)-2H-chromene-3-carboxamide 403/449 PET control N/A N/A [34]
PL-1 Peroxy Lucifer 1 410/475, 540 Boronate-deprotection mechanism; Ratiometric imaging Yes N/A [35]
RPF-1 Ratio Peroxyfluor 1 420/464, 517 Boronate-deprotection mechanism; Ratiometric imaging N/A N/A [36]
PF-1 Peroxyfluor-1 450/>460 Boronate-deprotection mechanism Yes N/A [31, 37]
PF-2 Peroxyfluor-2 475/511 Boronate-deprotection mechanism Yes Yes [25, 38, 39]
PF6-AM Peroxyfluor-6 acetoxymethyl ester 482/517 Boronate-deprotection mechanism; High cellular permeability Yes N/A [2, 25, 28]
NBzF N/A 490/525 PET control Yes N/A [40]
PF-3 Peroxyfluor-3 492/515 Boronate-deprotection mechanism Yes N/A [38]
RF-1 Redoxfluor 1 495/503 Boronate-deprotection mechanism Yes N/A [41]
MitoPY1 Mitochondrial Peroxy Yellow 1 510/528 Boronate-deprotection mechanism; Mitochondria-targeted Yes N/A [25, 42, 43]
PY1-ME Peroxy Yellow 1 Methyl-Ester 515/540 Boronate-deprotection mechanism Yes N/A [44]
(NucPE) 1 Nuclear Peroxy Emerald 1 514/540 Boronate-deprotection mechanism; Nuclear localized Yes Yes [45]
PY1 Peroxy Yellow 1 519/548 Boronate-deprotection mechanism Yes N/A [25, 38]
PO1 Peroxy Orange 1 540/565 Boronate-deprotection mechanism Yes N/A [25, 38]
Amplex Red Amplex Red 571/581 Conjunction with horseradish peroxidase N/A Yes [1, 46]
PR-1 Peroxy Red 1 ~575/585 Boronate-deprotection mechanism Yes N/A [31]
PCL-1 Peroxy Caged Luciferin 1 NA/612 Boronate-deprotection mechanism; Bioluminescence imaging N/A Yes [30, 47]
PCL-2 Peroxy Caged Luciferin 2   Boronate-deprotection mechanism; Bioluminescence imaging N/A Yes [48]
QCy7 Quinone Cy-7 595/635 Cyanine-based probe N/A Yes [49]
PN1 Peroxy Naphthalene 1 TPM750nm Boronate-deprotection mechanism N/A Yes [50]
SHP-Mito N/A TPM750nm Boronate-deprotection mechanism Mitochondria-targeted Yes Yes [51]