Fluorescence imaging of intracellular H
production using fluorescence probe PF6-AM (green). (A) Mechanism of Chemoselective H2O2 PF6-AM. (B) TPF imaging of H2O2 in astrocytes, fluorescence excited with a 770 nm Ti:sapphire laser. (C) Confocal microscopy of H2O2 in same astrocytes imaged in panel B, fluorescence excited with a 488 nm laser. The nuclei were stained with Hoechst 33342 (blue).