Figure 7
Knockdown of miR-20b inhibits tumorigenicity of breast cancer cells in vivo . (A) Schematic diagram of the experimental process. (B) Effects of antagomir-20b on tumor formation in nude mouse xenograft model. Female BALB/c nude mice were inoculated with ZR-75-30 cells transfected with antagomir-20b or negative control (antagomir NC) on the right flank (n = 6 mice/group). Tumor volume (V) was monitored by measuring the length (L) and width (W) with vernier caliper and calculated with the formula V = (L × W2) × 0.5. After 24 days, the mice were sacrificed and the tumor samples were collected (up-panel). The lower panel shows the volume of the tumors. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01 when compared with corresponding negative control. (C) qRT-PCR analysis of miR-20b expression in tumor tissues. ***, P < 0.001. (D) PTEN expression was detected by immunohistochemistry.