Figure 1

Expression of miR-20b is associated with PTEN level. (A) qRT-PCR analysis of miR-20b expression in 23 paired breast tumor tissues (tumor) and their adjacent normal tissues (normal). ***, P < 0.001. (B) The expression of miR-20b was examined in nontumorigenic breast epithelial cells (MCF-10A) and breast cancer cell lines, including MCF-7, ZR-75-30, T-47D and SK-BR-3. The miR-20b expression was normalized by U6 expression. ***, P < 0.001 compared with MCF-10A. (C) Western blot analysis of PTEN protein expression between the breast tumor and normal tissues. N, normal breast tissue; T, breast tumor tissues. (D) Quantitative analysis of PTEN protein expression. PTEN expression was normalized by β-actin.