Figure 8

Treg cells inhibited the formation of clusters during the activation phase of 8.3 CD8⁺T cell activation. The figure shows the bright field images (100X) of CD8⁺ 8.3 T cells un-stimulated (A) or stimulated with specific IGRP peptide (B), or stimulated with specific IGRP peptide in the presence of Tregs (C) for 72 hours. Irradiated (3,000 Rad) splenocytes from NOD mice were used as APCs. The results are the representative of 3 different individual experiments with similar findings.