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Figure 4 | Cell & Bioscience

Figure 4

From: Evaluation of immunosuppressive function of regulatory T cells using a novel in vitro cytotoxicity assay

Figure 4

IL-2/anti-IL-2 mAb complexes significantly expand Tregs in both B6 and NOD mice. Female NOD mice and B6 mice (6–8 week old, n = 4/group) were left untreated or treated with IL-2/anti-IL-2mAb. On day 5 cells were isolated from lymphoid organs and labeled with anti-CD4, anti-CD8 and anti-CD25 antibodies then with anti-Foxp3 and anti-GITR antibodies and analyzed by FACS for % of Foxp3+ cells among CD4+ T cells in spleen and pancreatic lymph nodes (A-B), the MFI of GITR and Foxp3 on Tregs in different groups were also analyzed (C-D).

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