Table 5 List of antibodies used
Antibody | Source | Dilution | Antigen retrieval |
|---|---|---|---|
CK7 | Mouse monoclonal; Dako, Glostrup, Denmark | 1:50 | Citrate buffer, pH 6.0 |
EpCAM | Mouse monoclonal; Dako, Glostrup, Denmark | 1:200 | Citrate buffer, pH 6.0 |
CD133 | Rabbit monoclonal; Cell Signaling Technology, Danvers, MA | 1:100 | Citrate buffer, pH 6.0 |
CD117 (c-kit) | Rabbit polyclonal; Dako, Glostrup, Denmark | 1:400 | EDTA buffer, PH 8.0 |
OV6 | Mouse monoclonal; R & D Systems, Emeryville, CA | 1:40 | EDTA buffer, PH 8.0 |
PCNA | Mouse monoclonal; Cell Signaling Technology, Danvers, MA | 1:4000 | Citrate buffer, pH 6.0 |