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Table 5 List of antibodies used

From: Proliferative ductular reactions correlate with hepatic progenitor cell and predict recurrence in HCC patients after curative resection

Antibody Source Dilution Antigen retrieval
CK7 Mouse monoclonal; Dako, Glostrup, Denmark 1:50 Citrate buffer, pH 6.0
EpCAM Mouse monoclonal; Dako, Glostrup, Denmark 1:200 Citrate buffer, pH 6.0
CD133 Rabbit monoclonal; Cell Signaling Technology, Danvers, MA 1:100 Citrate buffer, pH 6.0
CD117 (c-kit) Rabbit polyclonal; Dako, Glostrup, Denmark 1:400 EDTA buffer, PH 8.0
OV6 Mouse monoclonal; R & D Systems, Emeryville, CA 1:40 EDTA buffer, PH 8.0
PCNA Mouse monoclonal; Cell Signaling Technology, Danvers, MA 1:4000 Citrate buffer, pH 6.0
  1. CK 7 cytokeratin 7, EpCAM epithelial cell adhesion molecule , CD 133 cluster of differentiation (CD)133, CD117 (c-kit) mast/stem cell growth factor receptor, OV 6 oval cell marker, PCNA proliferating cell nuclear antigen.