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Table 1 The list of plasmids constructed and used in the study

From: Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58

Plasmid

Characteristics

pXHW54

RCA product from HPV58 CNZJ-3 (sample 9) was amplifiedwith Pr3506 (oYL35) and Pr7036 (oXHW262), gel purified andcloned into pCR-XL-TOPO vector. Insertion was verified bysequencing.

pXHW55

RCA product from HPV58 CNZJ-2 (sample 10) was amplifiedwith Pr3506 (oYL35) and Pr7036 (oXHW262), gel purified andcloned into pCR-XL-TOPO vector. Insertion was verified by sequencing.

pXHW56

RCA product from HPV58 CNZJ-1 (sample 13) was amplified with Pr3506 (oYL35) and Pr7036 (oXHW262), gel purified and cloned into pCR-XL-TOPO vector. Insertion was verified by sequencing.

pXHW57

RCA product from HPV58 CNZJ-3 (sample 9) was amplified with Pr6906 (oXHW263) and Pr3694 (oXHW264), gel purified and cloned into pCR-XL-TOPO vector. Insertion was verified by sequencing.

pXHW58

RCA product from HPV58 CNZJ-2 (sample 10) was amplified with Pr6906 (oXHW263) and Pr3694 (oXHW264), gel purified and cloned into pCR-XL-TOPO vector. Insertion was verified by sequencing.

pXHW59

RCA product from HPV58 CNZJ-1 (sample 13) was amplified with Pr6906 (oXHW263) and Pr3694 (oXHW264), gel purified and cloned into pCR-XL-TOPO vector. Insertion was verified by sequencing.

  1. Two bacterial colonies were picked up for each plasmid preparation and the obtained plasmid preps were named as a plasmid-1 or -2 (such as pXHW54-1 or -2 described in the text).