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Figure 2 | Cell & Bioscience

Figure 2

From: Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58

Figure 2

Nucleotide substitutions identified in HPV58 isolates to the reference HPV58 [32] by RCA-seq and by cloning sequencing. RCA-seq results were compared to the sequencing results of two individual bacterial clones of each plasmid for samples 9 (A), 10 (B), and 13 (C). S1 and S2 denotes Sanger sequence #1 (clone #1) and #2 (clone #2), respectively. Common nucleotide substitutions at positions in the reference HPV58 genome seen from RCA-seq to cloning sequencing were colored in red. The nucleotide substitutions identified only by cloning sequencing were shown in black.

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Cell & Bioscience

ISSN: 2045-3701

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