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Figure 1 | Cell & Bioscience

Figure 1

From: Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58

Figure 1

Enrichment of HPV genomic DNA by RCA from cervical samples. (A) HPV58 genome copy numbers before and after RCA enrichment. Real-time PCR (qPCR) was performed with an HPV58-specific primer pair on ~100 pg of sample DNA (sample 10 and sample 13) either before or after RCA enrichment. A 10-fold serial dilution, starting from 100 pg (~1.3 x 107 copies) of the plasmid pXW59-1 which contains an HPV58 DNA fragment from nt 6906 to 3695 was amplified using the same primer set by qPCR to create a standard curve. The threshold cycle (Ct) values of qPCR data from 2 repeats were calculated for copy number analysis. GAPDH was used as an internal control. (B) HPV58 DNA in the sample 10 was under detection level before RCA, but became detectable by agarose gel electrophoresis after enrichment by RCA to 20481 copies as quantified by qPCR.

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Cell & Bioscience

ISSN: 2045-3701

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