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Figure 5 | Cell & Bioscience

Figure 5

From: Peptidylarginine deiminase IV promotes the development of chemoresistance through inducing autophagy in hepatocellular carcinoma

Figure 5

Inhibition of autophagy restored the sensitivity of HCC cells to chemotherapy. (A) SMMC-7721 and Hep-G2 cells (1 × 104/well) that overexpressed PADI4 were cultured in a 96-well plate with an existence of 5-Fu (120 μg/mL) for 24 hours. The occurrence of autophagy was inhibited by autophagy inhibitor-3-MA or sh-Atg7 and sh-Beclin1. MTT was used to detect the viability of the cells. (B) PADI4 was overexpressed in SMMC-7721 cells. The occurrence of autophagy was inhibited by sh-Atg7. Then the cells (5 × 106) were performed subcutaneous administration in the nude mice armpit area. After implantation, recipients were injected in tumor in situ with 5-Fu (30 mg/kg) every 3 days. After 27 days of implantation, the animals were sacrificed and tumors were dissected. The tumor weight and volume of each groups were measured after been removed from the mice. (*Compared with the group that untreated with chemotherapy drugs, P < 0.05; #Compared with the group that treated with chemotherapy drugs, P < 0.05)

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