Figure 2
PADI4 enhanced chemoresistance of hepatocellular carcinoma cells in vitro. (A) SMMC-7721 cells were treated with 5-Fu (120 μg/mL) with overexpression of PADI4 or not. The morphology of the cells was observed by microscope. (B) PADI4 was overexpressed in SMMC-7721 or HepG2 cells and the cells (1 × 104/well) were cultured in a 96-well plate with an existence of 5-Fu (120 μg/mL) or cisplatin (8 μg/mL) for 24 hours. MTT was employed to examine the viability of SMMC-7721 or Hep-G2 cells. (C) PADI4 was overexpressed in SMMC-7721 cells and the cells were cultured in a 6-well plate with an existence of 5-Fu (120 μg/mL) for 24 hours. Flow cytometry was used to measure apoptosis of the cells. (*Compared with the group that untreated with chemotherapy drugs, P < 0.05; #Compared with the group that treated with chemotherapy drugs, P < 0.05)