Figure 1

SIRT1, VEGF-C and D2-40 expression in esophageal tissue samples. (A) Immunohistochemical staining of SIRT1, VEGF-C protein, lymphatic microvessels and lymphovascular invasion in pN0 ESCC tissues: (a) SIRT1 positive expression; (b) SIRT1 negative expression (magnification × 400); (c) VEGF-C protein positive expression; (d) VEGF-C protein negative expression (magnification × 400); (e) lymphatic microvessel labeled with D2-40 in peritumoral stromal tissue (magnification × 200); (f) lymphovascular invasion highlighted by D2-40 (magnification × 400). (B) SIRT1 protein expression was evaluated by western blotting in ESCC tissues and paired noncancerous tissues.