Figure 5

Autophagy protects hepatocytes from cholestatic injury by eliminating ROS. (A) L02 normal liver cells were incubated with GCDC for 24 h with 10 µM CQ. Cellular ROS activity was determined using DCF-DA staining. (B) Immunohistochemical staining of 8-OhdG in the liver of mice. (C) L02 normal liver cells were pretreated with 10 mM NAC for 2 h, and cells were incubated in GCDC (200 µM) or with 10 µM CQ for the indicated time. Cell viability was assessed by MTT assay. Data (mean ± SD) were determined in at least three independent experiments. (*: P < 0.05). (D) Mice were treated as indicated and serum levels of ALT and AST were determined. Data were expressed as mean ± SD. (**: P < 0.01).