Bile duct ligation leads to liver injury and activation of autophagy. (A) Plasma ALT, AST and TBIL levels were analyzed in mice treated with either BDL or control after 2 weeks. Data were expressed as mean ± SD. (*: P < 0.05). (B) Representative liver sections were collected from the mice of BDL and control group, then protein levels of LC3 of liver samples were analyzed by an immunoblot assay. (C) The liver samples were processed for electron microscope and representative electron micrographs were shown. Arrows denote autophagosomes. (N: nucleus; Bar: 20 µm). (D) Data were derived from three independent experiments. (*: P < 0.05).