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Figure 4 | Cell & Bioscience

Figure 4

From: Pinocembrin suppresses TGF-β1-induced epithelial-mesenchymal transition and metastasis of human Y-79 retinoblastoma cells through inactivating αvβ3 integrin/FAK/p38α signaling pathway

Figure 4

The inhibitory effect of pinocembrin on TGF-β1-induced activities and expressions of MMP-2/-9 in Y-79 cells. The cultured cells in serum-free medium were pretreated with TGF-β1 (10 ng/ml) and then incubated in the different concentrations of pinocembrin (0, 1, 2.5, and 5 μM). (A) The conditioned media were collected and MMP-2/-9 activities were determined by gelatin zymography. (B) The MMP-2/-9 protein expressions were analyzed by Western blotting. (C) The MMP-2/-9 mRNA expressions were analyzed by RT-PCR. β-Actin and GADPH was used as internal controls.

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Cell & Bioscience

ISSN: 2045-3701

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