Figure 4
RNF39 protein effects on HIV-1 replication in Jurkat cells as evaluated by RNA interference assay. A. Flowchart depicting siRNA effect on the replication of retroviruses (HIV-1 molecular clone pNL4-3 and HTLV-1 molecular clone k30). B. Inhibition of HIV-1 replication by RNF39 downregulation in Jurkat cells, measured using western blot analysis. C. Reduction of HIV-1 p24 antigen levels in culture supernatants from siRNF39-transfected Jurkat cells compared with culture supernatants from siNC-transfected cells, measured using HIV-1 p24 ELISA. D. No significant reduction of HTLV-1 p19 antigen levels was observed in culture supernatants from siRNAs-transfected Jurkat cells, measured using HTLV-1 p19 ELISA.