RNF39 protein effects on HIV-1 replication in 293 T cells as evaluated by cDNA overexpression technique. A. RNF39 mRNA overexpression in 293 T cells by using the RNF39 cDNA vector under CMV promoter control. RNF39 relative mRNA expression was quantified by qPCR. Values are normalized to those of pCMV-transfected cells. Data represent the mean ± SD of three independent experiments. B. Enrichment of HIV-1 infection via RNF39 overexpression in 293 T cells. C. Enrichment of HIV-1 infection via RNF39 overexpression in siRNF39-pZNRD1- and siRNF39-pRNF39-transfected 293 T cells.