Unsaturated fatty acid (UFA) concentration and SCD-1 expression. (A) C16:1n-7/C16:0 and C18:1n-9/C18:0 ratios were compared and used as surrogate measures of hepatic SCD-1 activity. n = 5 in each group (B) RT-PCR analysis of SCD-1 gene expression in mouse liver from control group (Ctrl), hypothyroid group (Hypo) and T3-treated group (T3). 18s amplified in parallel served as internal reference. n = 6 in each group. (C) Western blotting analysis of SCD-1 with β-Actin as an indication of equal protein loading. (D) Fatty acids were grouped according to the degree of saturation and the amount of fatty acid belonging to the same group was added up. The relative contents of each group were compared across the Ctrl, Hypo and T3. n = 5 in each group. (E) The ratios between n-6 PUFA and n-3 PUFA in different groups were compared. n = 5 in each group. *P < 0.05 vs. Ctrl, ‡P < 0.05 vs. Hypo.