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Figure 8 | Cell & Bioscience

Figure 8

From: Finding of widespread viral and bacterial revolution dsDNA translocation motors distinct from rotation motors by channel chirality and size

Figure 8

Single molecule polarization detection to investigate motor rotation. (A) Experiment design of single molecule polarization detection on motor pRNA rotation during DNA packaging. The motor was stalled by γ-S-ATP and the rotation of pRNA ring can be excluded since no anti-correlated signals of a single Cy3 fluorophore in horizontal (H) or vertical (V) channels were observed. (B and C) Typical time trajectories of Cy3 fluorescence intensity in horizontal (black) and vertical (red) channels without (B) and with (C) the addition of ATP to restart the packaging.

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