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Figure 3 | Cell & Bioscience

Figure 3

From: Finding of widespread viral and bacterial revolution dsDNA translocation motors distinct from rotation motors by channel chirality and size

Figure 3

Demonstration of Phi29 connector channel size larger than the diameter of dsDNA as proved by single pore translocation of double-stranded and tetra-stranded DNA by conductance assay. Current blockage of the channel by double-stranded was 32% (A) and by tetra-stranded DNA was 64% (B). Purified Phi29 connector was incorporated into lipid bilayer. Switch of voltage polarity revealed that the channel allowed only unidirectional translocation of both dsDNA (A) and tetra-stranded DNA (B).

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