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Figure 5 | Cell & Bioscience

Figure 5

From: Comparative analysis of the activation of unfolded protein response by spike proteins of severe acute respiratory syndrome coronavirus and human coronavirus HKU1

Figure 5

Impact of N-linked glycosylation on UPR activation by S proteins. (A, B) N-linked glycosylation mutants. SARS-CoV and HCoV-HKU1 S proteins and their N-linked glycosylation mutants (SCV-Sm and HKU1-Sm) were expressed in 293FT cells. To remove N-linked glycans, cell lysates were incubated with endoglycosidase PNGase F for 1 h at 37°C. (C-F) Luciferase reporter assay. S proteins and their N-linked glycosylation mutants in escalating dose were compared for the activity to activate luciferase reporter expression driven by Grp78 and Grp94 promoters.

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