Figure 3

MiR-206 suppresses anchorage-dependent GC cell growth and xenograft tumor formation. (A) The expression level of miR-206 in P2GM-miR-206 stable cell line and vector-carrying control stable cells (***P < 0.0001). (B) One thousand stable SGC-7901 cells of miR-206 and negative control were plated onto 6-well plates and a colony formation assay carried out. Representative results of colony formation of P2GM-miR-control, P2GM-miR-206. (C) The number of colonies was counted on the 14th day after seeding. Stable SGC-7901 cells of miR-206 grew to a lower density compared to NC. The results represent the means ± SEM of three independent experiments (**P < 0.01). (D) SGC-7901 cells with stable expression of miR-206 or not were inoculated subcutaneously into both flanks of nude mice(n = 5 per group). Representative photographs of nude mice 24 days after inoculation are shown. (E) The mice were killed and the tumors were weighted 24 days after inoculation, the xenografts with miR-206 overexpression were significantly smaller than the control xenografts. (F) shows tumor growth curve (*P < 0.05 and **P < 0.01). (G) Tumors from each group were weighed immediately after removal. The average tumor weight is indicated as means ± SEM (*P < 0.05).