Figure 6

SRC-3 deficiency leads to increased activation of IKK-IκB-NF-κB pathway. BMMCs were stimulated with anti-DNP IgE 1 μg/ml for 4 h, and then treated with DNP-HSA 10 ng/ml for various times. Whole cell lysates were analyzed by western-blotting for IKK, p-IKK, IκB, p-IκB, p65 and p-p65. Representative images are shown from three independent experiments.