Passive cutaneous anaphylaxis in SRC-3+/+and SRC-3-/-mice. SRC-3+/+ (n = 6) and SRC-3-/- mice (n = 6) were sensitized with anti-DNP IgE and DNP-HSA to induce cutaneous anaphylaxis as described in methods (A-D). A, dye extravasation was observed after DNP-HSA injection at the injection sites in the ears. Photographs of the mice were taken 90 min after DNP-HSA administration. Representative images are shown. B, Extravasation of Evan’s blue was quantified as described in methods. Values are expressed as means + SD from three independent experiments. C, Toludine blue staining of mast cells in the ear skin of SRC-3+/+ and SRC-3-/- mice after antigen challenge. Representative images are shown; arrows indicate degranulated tissue mast cells. D, mast cells were quantified, values are expressed as means + SD from three independent experiments.