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Figure 4 | Cell & Bioscience

Figure 4

From: Activation of Akt protects cancer cells from growth inhibition induced by PKM2 knockdown

Figure 4

Inhibition of PI3K-Akt signaling pathway induces growth inhibition and cell death in Si-PKM cells. (A) Si-PKM cells in 6-well dish were treated with: DMSO, 1 μM Wortmannin, 1 μM AKTi, 100 nM Rapamycin and 5 mM 3-MA respectively for 2 hours. Cell lysates were then analyzed by Western blot with antibodies against p-Akt and total Akt. GAPDH was used as an equal loading control. (B) Si-C and Si-PKM cells were treated with 10 μM LY294002 for 24 hours or left untreated, Cell lysates were analyzed by Western blot with antibodies against p-Akt and total Akt, GAPDH was used as an equal loading control. (C) Si-C and Si-PKM cells in 96-well plate were treated with 10 μM LY294002 or left untreated, plates were subjected to MTT assay after 24 h. Reactions were carried out in triples. Data are shown as means ± SEM. n = 3. Statistical analyses were carried out using Student’s t-test. Significance: *p < 0.05; **p < 0.001. (D) Si-C and Si-PKM cells in 6 well plate were treated with 10 μM LY294002 for 24 hours or left untreated, cell lysates were analyzed by Western blot with antibodies against PARP. GAPDH was used as an equal loading control. Similar results were obtained in three independent experiments. Representative data are shown.

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