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Figure 3 | Cell & Bioscience

Figure 3

From: Activation of Akt protects cancer cells from growth inhibition induced by PKM2 knockdown

Figure 3

Activation of Akt is a result of glycolysis disruption. (A) Using a lentiviral protein expression vector, we reconstituted the expression of PKM1 or PKM2 in Si-PKM cells. Cell lysates were analyzed by immunoblotting with the following antibodies: PKM1/2, PKM2 and Flag. GAPDH was used as an equal loading control. (B) Re-expression of either PKM2 or PKM1 normalized p-Akt to similar level as that of Si-C cells. (C) Si-C cells, Si-PKM cells, PKM1 cells and PKM2 cells were kept in high glucose DMEM or glucose-null DMEM for 2 hours, cell lysates were analyzed by immunoblotting with antibodies against p-Akt, total Akt and GAPDH. Similar results were obtained in three independent experiments. Representative data are shown. (D) The p-Akt levels were normalized to the loading control and presented as relative conversion to values in Si-C cells with high glucose DMEM. Data are shown as means ± SEM. n = 3. Statistical analyses were carried out using Student’s t-test. Significance: *p < 0.05; **p < 0.001. (E) Si-C cells, Si-PKM cells, PKM1 cells and PKM2 cells were treated with 25 mmol/L 2-DG or left untreated for 2 hours, cell lysates were analyzed by immunoblotting with antibodies against p-Akt, total Akt and GAPDH. Similar results were obtained in three independent experiments. Representative data are shown.

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