Figure 4
The effect of PTEN overexpression on differentiation and collagen secretion of LPS-induced lung fibroblasts. Cellular expression of α-SMA examined by Western blot (A) and PICP content in cell culture supernatants detected by ELISA (B) were used to reflect the effect of overexpression of PTEN on lung fibroblast differentiation and collagen secretion 72 h after 1 μg/mL LPS challenge. bpV(phen)(1 μM for 0.5 h) was used to investigate the effect of overexpression of PTEN on lung fibroblast differentiation and collagen secretion. PI3-K inhibitor Ly294002 (50 μmol/L for 1 h) was used to assess the effect of PTEN overexpression and PI3-K-Akt pathway inhibition on lung fibroblast differentiation and collagen secretion in the presence or absence of LPS. *p < 0.05 vs. untreated control group; †p < 0.05 vs. PTEN group; ‡p < 0.05 vs. PTENLPS group. §p < 0.05 vs. PTEN group; ||p < 0.05 vs. PTENLPS group. Columns represent mean values and error bars represent SD (Mean±SD). Blots are representative of three independent experiments.