The effect of PTEN overexpression on activation of PI3-K-Akt-GSK3β pathway in lung fibroblasts. Cellular protein was collected from lung fibroblasts treated with 1 μM bpV(phen) for 0.5 h before exposure of the cells to LPS and transfected with PTEN overexpression vector for up to 72 h. Afterwards, the total and phosphor-Akt (Ser473) (2A) and GSK3β (2B) were detected by Western Blot. *p < 0.05 vs. Blank and Empty group; †p < 0.05 vs. PTEN group; ‡p < 0.05 vs. PTENLPS group. §p < 0.05 vs. PTEN group; ||p < 0.05 vs. PTENLPS group. Columns represent mean values and error bars represent SD. Blots are representative of three independent experiments.