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Figure 1 | Cell & Bioscience

Figure 1

From: Overexpression of PTEN suppresses lipopolysaccharide-induced lung fibroblast proliferation, differentiation and collagen secretion through inhibition of the PI3-K-Akt-GSK3beta pathway

Figure 1

Expression and dephosphorylation activity of PTEN in lung fibroblasts transfected with PTEN overexpression lentivirus. The total RNA and cellular protein were collected from lung fibroblasts transfected with PTEN overexpression lentivirus (5 × 104 TU/mL) for 48 h and treated with bpV(phen)(1 μM) for 0.5 h before exposure of the cells to 1 μg/mL LPS for up to 72 h, followed by detecting PTEN mRNA, protein expression and dephosphorylation activity by real-time RT-PCR (1A), Western blot (1B) and Malachite green-based assay (1C). *p < 0.05 vs. Blank and Empty group; p < 0.05 vs. PTEN group; p < 0.05 vs. PTENLPS group. Columns represent mean values and error bars represent SD. Blots are representative of three independent experiments.

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