Table 1 The binding affinities of wild-type BmKKx2 and its mutants towards hERG channel
From: Open conformation of hERG channel turrets revealed by a specific scorpion toxin BmKKx2
BmKKx2 mutant | IC50a | n b | IC50(mut)/IC50(wt) |
|---|---|---|---|
nM | |||
Wild type | 26.7 ± 1.7 | 6 | 1.0 |
R1A | 238.3 ± 11.2 | 4 | 8.1 |
Y11A | 131.0 ± 35.2 | 4 | 27.9 |
K18A | 376.6 ± 62.0 | 6 | 80.1 |
R20A | 409.6 ± 56.9 | 5 | 87.1 |
F21A | 863.4 ± 73.4 | 5 | 183.7 |
K23A | 123.2 ± 56.3 | 4 | 26.2 |
R27A | 262.7 ± 25.9 | 4 | 13.3 |
F32A | 24.1 ± 1.9 | 4 | 0.9 |