Figure 1
From: Open conformation of hERG channel turrets revealed by a specific scorpion toxin BmKKx2
Purification and characterization of toxin BmKKx2. (A) Amino acid sequence alignment for BmKKx2 and BeKm-1. The differential residues were marked with asterisks. (B) Tricine-SDS-PAGE analysis of purified toxin BmKKx2. Lane 1, molecular mass markers; lane 2, non-induced cell-free extract of E. coli carrying pGEX-6p-1-BmKKx2; Lane 3, total cell-free extract of E. coli carrying pGEX-6p-1-BmKKx2 induced with IPTG for 4 h; lane 4, the purified GST-BmKKx2 protein after affinity chromatography; lane 5, the purified GST fusion protein after desalting and concentration; lane 6, the cleaved fusion protein via enterokinase; lane 7, the purified toxin BmKKx2 through reversed phase HPLC. (C) HPLC profile of the GST-BmKKx2 fusion protein cleaved through enterokinase. (D) Mass spectrum of BmKKx2 peptide measured on MALDI-TOF-MS.