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Table 1 Difference of transfection rate  x ¯ ± s

From: Expression and characterization of a soluble VEGF receptor 2 protein

 

Control group (n = 5)

Truncated- rVegfr2 2 0.05 group (n = 5)

Truncated-rVegfr2 0.l0 group (n = 5)

Truncated- rVegfr2 0.l5gourp (n = 5)

rVegfr2 0.05group (n = 5)

rVegfr2 0.l0group (n = 5)

rVegfr2 0.l5group (n = 5)

Green fluorescent points

303.2 ± 19.1

324.8 ± 28.5

387.2 ± 14.5*

216.0 ± 23.3*

272.2 ± 13.1

261.8 ± 17.0*

142.4 ± 15.8*

Red fluorescent points

532.2 ± 17.8

452.4 ± 54.6

311.2 ± 20.5

446.6 ± 44.2*

307.8 ± 34.1*

443.0 ± 48.6*

149.2 ± 35.6*

Blue fluorescent points

1272.0 ± 49.5

1593.6 ± 116.6*

1640.0 ± 256.9

1252.0 ± 151.3

1029.6 ± 76.9

932.0 ± 55.8*

1030.4 ± 135.9

Transfection rate of GFP (%)

16.8 ± 0.8

15.9 ± 1.6

20.1 ± 2.5

12.8 ± 2.3

20.4 ± 1.6

19.1 ± 2.1

12.3 ± 2.3*

Apoptotic index (%)

29.5 ± 1.0

22.1 ± 2.2*

16.2 ± 2.6*

26.4 ± 3.0

23.1 ± 3.1*

32.2 ± 2.7

12.8 ± 3.2*

  1. *P < 0.05(Compared with the normal control group) cells were stained with PI and Hoechst.
  2. Green fluorescent points: the number of pCMV-gfp-transfected HEK293 cells.
  3. Transfection efficiency: the number of green fluorescence-positive cells/ the number of living cells (Hoechst-stained cells).
  4. Apoptosis index: the number of PI-positive cells/the number of Hoechst-stained cells.
  5. rVegfr2: Rat-Vegfr2.
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