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Table 1 Difference of transfection rate  x ¯ ± s

From: Expression and characterization of a soluble VEGF receptor 2 protein

  Control group (n = 5) Truncated- rVegfr2 2 0.05 group (n = 5) Truncated-rVegfr2 0.l0 group (n = 5) Truncated- rVegfr2 0.l5gourp (n = 5) rVegfr2 0.05group (n = 5) rVegfr2 0.l0group (n = 5) rVegfr2 0.l5group (n = 5)
Green fluorescent points 303.2 ± 19.1 324.8 ± 28.5 387.2 ± 14.5* 216.0 ± 23.3* 272.2 ± 13.1 261.8 ± 17.0* 142.4 ± 15.8*
Red fluorescent points 532.2 ± 17.8 452.4 ± 54.6 311.2 ± 20.5 446.6 ± 44.2* 307.8 ± 34.1* 443.0 ± 48.6* 149.2 ± 35.6*
Blue fluorescent points 1272.0 ± 49.5 1593.6 ± 116.6* 1640.0 ± 256.9 1252.0 ± 151.3 1029.6 ± 76.9 932.0 ± 55.8* 1030.4 ± 135.9
Transfection rate of GFP (%) 16.8 ± 0.8 15.9 ± 1.6 20.1 ± 2.5 12.8 ± 2.3 20.4 ± 1.6 19.1 ± 2.1 12.3 ± 2.3*
Apoptotic index (%) 29.5 ± 1.0 22.1 ± 2.2* 16.2 ± 2.6* 26.4 ± 3.0 23.1 ± 3.1* 32.2 ± 2.7 12.8 ± 3.2*
  1. *P < 0.05(Compared with the normal control group) cells were stained with PI and Hoechst.
  2. Green fluorescent points: the number of pCMV-gfp-transfected HEK293 cells.
  3. Transfection efficiency: the number of green fluorescence-positive cells/ the number of living cells (Hoechst-stained cells).
  4. Apoptosis index: the number of PI-positive cells/the number of Hoechst-stained cells.
  5. rVegfr2: Rat-Vegfr2.